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canine ifnγ elispot development module (R&D Systems)

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R&D Systems canine ifnγ elispot development module

(A) Total IgG titers against RABV-G and RABV-N on the day of a rabies vaccine booster (Day 0) or 7-14 days after vaccine administration. The dashed line was determined by the antibody titer from an unvaccinated puppy (Dog 11, ) and was used as a normalization control for each experiment ( n = 10, paired two-tailed Wilcoxon test; ns, not significant, ** p < 0.01; two independent experiments). (B) Representative <t>IFNγ</t> <t>ELISpot</t> images of mock-, RABV-N-, and RABV-G- stimulated PBMCs performed in triplicate. (C) Quantification of the data represented in (B) ( n = 6; one-way ANOVA and post hoc Tukey’s test; ns, not significant, * p < 0.05, ** p < 0.01; three independent experiments).

Canine Ifnγ Elispot Development Module, supplied by R&D Systems, used in various techniques. Bioz Stars score: 93/100, based on 4 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/canine+ifn%CE%B3+elispot+development+module/Canine+IFN-gamma+ELISpot+Development+Module%2C+5+Plate/pmc12068608-66-1-7
Average 93 stars, based on 4 article reviews

canine ifnγ elispot development module - by Bioz Stars, 2026-10

93/100 stars

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1) Product Images from "Rabies vaccination induces a CD4 + T EM and CD4 + CD8 + T EMRA T H 1 phenotype in dogs"

Article Title: Rabies vaccination induces a CD4 + T EM and CD4 + CD8 + T EMRA T H 1 phenotype in dogs

Journal: PLOS One

doi: 10.1371/journal.pone.0323823

Figure Legend Snippet: (A) Total IgG titers against RABV-G and RABV-N on the day of a rabies vaccine booster (Day 0) or 7-14 days after vaccine administration. The dashed line was determined by the antibody titer from an unvaccinated puppy (Dog 11, ) and was used as a normalization control for each experiment ( n = 10, paired two-tailed Wilcoxon test; ns, not significant, ** p < 0.01; two independent experiments). (B) Representative IFNγ ELISpot images of mock-, RABV-N-, and RABV-G- stimulated PBMCs performed in triplicate. (C) Quantification of the data represented in (B) ( n = 6; one-way ANOVA and post hoc Tukey’s test; ns, not significant, * p < 0.05, ** p < 0.01; three independent experiments).

Techniques Used: Control, Two Tailed Test, Enzyme-linked Immunospot

(A) Representative histogram plots compare the mock control (black outline) to RABV-G-stimulated T cell subsets: CD4 + (top row, blue), DP (middle row, orange), and CD8 + (bottom row, black), with % proliferated (CTFR LO ) shown within the plot. (B) Quantification of (A) (mean ± SEM; n = 6/experiment; two-way ANOVA with multiple comparisons **** p < 0.0001; 6 independent experiments). Data was normalized to controls. (C) Representative histograms of IFNγ (left) and IL-17A (right) expression by CD8 + , CD4 + , or DP T cells stimulated with RABV-G after 5 days. (D) Mean fluorescence intensity (MFI) of IFNγ (left) and IL-17A (right) for CD4 + T cells, DP T cells, and CD8 + T cells (mean ± SEM; n = 6/experiment; two-way ANOVA with multiple comparisons; ns, not significant, * p < 0.05, *** p < 0.001, **** p < 0.0001; 6 independent experiments). MFI was normalized to controls.

Figure Legend Snippet: (A) Representative histogram plots compare the mock control (black outline) to RABV-G-stimulated T cell subsets: CD4 + (top row, blue), DP (middle row, orange), and CD8 + (bottom row, black), with % proliferated (CTFR LO ) shown within the plot. (B) Quantification of (A) (mean ± SEM; n = 6/experiment; two-way ANOVA with multiple comparisons **** p < 0.0001; 6 independent experiments). Data was normalized to controls. (C) Representative histograms of IFNγ (left) and IL-17A (right) expression by CD8 + , CD4 + , or DP T cells stimulated with RABV-G after 5 days. (D) Mean fluorescence intensity (MFI) of IFNγ (left) and IL-17A (right) for CD4 + T cells, DP T cells, and CD8 + T cells (mean ± SEM; n = 6/experiment; two-way ANOVA with multiple comparisons; ns, not significant, * p < 0.05, *** p < 0.001, **** p < 0.0001; 6 independent experiments). MFI was normalized to controls.

Techniques Used: Control, Expressing, Fluorescence

(A) Gating strategy and representative contour plots for lineage-defining subsets between CTFR LO and CTFR HI T cells to differentiate T H 1 (T-bet + IFNγ + ), T H 17 (IL-17A + ), T H 1/T H 17 (T-bet + IL-17A + ), pathogenic T H 1/T H 17 (T-bet + IL-17A + IFNγ + ), and Tregs (FOXP3 + ). (B) Quantification of (A) (mean ± SEM, n = 22 samples, paired two-tailed Wilcoxon test; * p < 0.05, *** p < 0.001, **** p < 0.0001; 4 independent experiments).

Figure Legend Snippet: (A) Gating strategy and representative contour plots for lineage-defining subsets between CTFR LO and CTFR HI T cells to differentiate T H 1 (T-bet + IFNγ + ), T H 17 (IL-17A + ), T H 1/T H 17 (T-bet + IL-17A + ), pathogenic T H 1/T H 17 (T-bet + IL-17A + IFNγ + ), and Tregs (FOXP3 + ). (B) Quantification of (A) (mean ± SEM, n = 22 samples, paired two-tailed Wilcoxon test; * p < 0.05, *** p < 0.001, **** p < 0.0001; 4 independent experiments).

Techniques Used: Two Tailed Test

Enzyme-linked Immunospot:

Article Title: Rabies vaccination induces a CD4+ TEM and CD4+CD8+ TEMRA TH1 phenotype in dogs.
Article Snippet: .. A canine IFNγ ELISpot Development Module (SEL781, R&D Systems) was used per the manufacturer’s protocol with slight modifications. .. Briefly, an 8-strip Immobilon-P® hydrophobic polyvinylidene fluoride (PVDF) membrane microplate (M8IPS4510, Millipore) was pre-wet with 35% ethanol, washed with PBS, coated with anti-canine IFNγ capture antibody (840804, R&D Systems), and incubated at 4°C overnight.

Article Title: Rabies vaccination induces a CD4 + T EM and CD4 + CD8 + T EMRA T H 1 phenotype in dogs
Article Snippet: .. A canine IFNγ ELISpot Development Module (SEL781, R&D Systems) was used per the manufacturer’s protocol with slight modifications. .. Briefly, an 8-strip Immobilon-P ® hydrophobic polyvinylidene fluoride (PVDF) membrane microplate (M8IPS4510, Millipore) was pre-wet with 35% ethanol, washed with PBS, coated with anti-canine IFNγ capture antibody (840804, R&D Systems), and incubated at 4°C overnight.

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Journal: PLOS One

Article Title: Rabies vaccination induces a CD4 + T EM and CD4 + CD8 + T EMRA T H 1 phenotype in dogs

doi: 10.1371/journal.pone.0323823

Figure Lengend Snippet: (A) Total IgG titers against RABV-G and RABV-N on the day of a rabies vaccine booster (Day 0) or 7-14 days after vaccine administration. The dashed line was determined by the antibody titer from an unvaccinated puppy (Dog 11, ) and was used as a normalization control for each experiment ( n = 10, paired two-tailed Wilcoxon test; ns, not significant, ** p < 0.01; two independent experiments). (B) Representative IFNγ ELISpot images of mock-, RABV-N-, and RABV-G- stimulated PBMCs performed in triplicate. (C) Quantification of the data represented in (B) ( n = 6; one-way ANOVA and post hoc Tukey’s test; ns, not significant, * p < 0.05, ** p < 0.01; three independent experiments).

Article Snippet: A canine IFNγ ELISpot Development Module (SEL781, R&D Systems) was used per the manufacturer’s protocol with slight modifications.

Techniques: Control, Two Tailed Test, Enzyme-linked Immunospot

Journal: PLOS One

Article Title: Rabies vaccination induces a CD4 + T EM and CD4 + CD8 + T EMRA T H 1 phenotype in dogs

doi: 10.1371/journal.pone.0323823

Figure Lengend Snippet: (A) Representative histogram plots compare the mock control (black outline) to RABV-G-stimulated T cell subsets: CD4 + (top row, blue), DP (middle row, orange), and CD8 + (bottom row, black), with % proliferated (CTFR LO ) shown within the plot. (B) Quantification of (A) (mean ± SEM; n = 6/experiment; two-way ANOVA with multiple comparisons **** p < 0.0001; 6 independent experiments). Data was normalized to controls. (C) Representative histograms of IFNγ (left) and IL-17A (right) expression by CD8 + , CD4 + , or DP T cells stimulated with RABV-G after 5 days. (D) Mean fluorescence intensity (MFI) of IFNγ (left) and IL-17A (right) for CD4 + T cells, DP T cells, and CD8 + T cells (mean ± SEM; n = 6/experiment; two-way ANOVA with multiple comparisons; ns, not significant, * p < 0.05, *** p < 0.001, **** p < 0.0001; 6 independent experiments). MFI was normalized to controls.

Article Snippet: A canine IFNγ ELISpot Development Module (SEL781, R&D Systems) was used per the manufacturer’s protocol with slight modifications.

Techniques: Control, Expressing, Fluorescence

Journal: PLOS One

Article Title: Rabies vaccination induces a CD4 + T EM and CD4 + CD8 + T EMRA T H 1 phenotype in dogs

doi: 10.1371/journal.pone.0323823

Figure Lengend Snippet: (A) Gating strategy and representative contour plots for lineage-defining subsets between CTFR LO and CTFR HI T cells to differentiate T H 1 (T-bet + IFNγ + ), T H 17 (IL-17A + ), T H 1/T H 17 (T-bet + IL-17A + ), pathogenic T H 1/T H 17 (T-bet + IL-17A + IFNγ + ), and Tregs (FOXP3 + ). (B) Quantification of (A) (mean ± SEM, n = 22 samples, paired two-tailed Wilcoxon test; * p < 0.05, *** p < 0.001, **** p < 0.0001; 4 independent experiments).

Article Snippet: A canine IFNγ ELISpot Development Module (SEL781, R&D Systems) was used per the manufacturer’s protocol with slight modifications.

Techniques: Two Tailed Test

Time course of T cell responses to GFP and capsid in peripheral blood mononuclear cells (PBMCs) and lymphocytes isolated from spleen and liver, determined by IFN-γ ELISpot assay. T cells were stimulated with GFP or peptide pools (A, B, C) corresponding to AAV2 and AAV8 capsid, respectively, and the number of IFN-γ-secreting cells in response to antigen stimulation was determined. T cell responses to GFP can be observed in the two animals that received AAV8 on day 27. SFC, spot-forming cells.

Journal: Human Gene Therapy

Article Title: Evaluation of Adeno-Associated Viral Vectors for Liver-Directed Gene Transfer in Dogs

doi: 10.1089/hum.2010.194

Figure Lengend Snippet: Time course of T cell responses to GFP and capsid in peripheral blood mononuclear cells (PBMCs) and lymphocytes isolated from spleen and liver, determined by IFN-γ ELISpot assay. T cells were stimulated with GFP or peptide pools (A, B, C) corresponding to AAV2 and AAV8 capsid, respectively, and the number of IFN-γ-secreting cells in response to antigen stimulation was determined. T cell responses to GFP can be observed in the two animals that received AAV8 on day 27. SFC, spot-forming cells.

Article Snippet: Enzyme-linked immunospot (ELISpot) assays to detect canine interferon (IFN)-γ were performed with the canine IFN-γ ELISpot development module and ELISpot blue color module (R&D Systems, Minneapolis, MN), in accordance with the manufacturer's instructions.

Techniques: Isolation, Enzyme-linked Immunospot

Development of T cells against transgene and capsid in animal S468 determined by IFN-γ ELISpot. Top: Time course of T cell frequencies in PBMCs against GFP and AAV8 capsid (peptide pools 8A, 8B, 8C; control, C). Bottom: Frequencies of GFP and capsid-specific T cells isolated from spleen and liver compared with PBMCs on day 35 after vector administration.

Journal: Human Gene Therapy

Article Title: Evaluation of Adeno-Associated Viral Vectors for Liver-Directed Gene Transfer in Dogs

doi: 10.1089/hum.2010.194

Figure Lengend Snippet: Development of T cells against transgene and capsid in animal S468 determined by IFN-γ ELISpot. Top: Time course of T cell frequencies in PBMCs against GFP and AAV8 capsid (peptide pools 8A, 8B, 8C; control, C). Bottom: Frequencies of GFP and capsid-specific T cells isolated from spleen and liver compared with PBMCs on day 35 after vector administration.

Techniques: Enzyme-linked Immunospot, Control, Isolation, Plasmid Preparation

Fig. 8A–C. Vaccination with insulin-pulsed NOD-DC1 cells induced a change in Th1/Th2 balance. At day 10 after CY- treatment IL-10 and IFN-γ expression was measured. (A) Cy- tokine expression in the pancreas detected by Light Cycler RT- PCR. The number of mRNA copies are expressed per 1×106 β- actin molecules. (B) Cytokine secretion in culture supernatants after stimulation of 3×106 splenocytes with 50 µg/ml insulin for 3 days. (C) ELISPOT analysis of insulin-specific T-cell re- activity in NOD mice following vaccination with NOD-DC-1 cells. The frequency of IFN-γ spot-forming colonies (SFC) per well in response to 50 µg/ml insulin were calculated from trip- licates of individual mice. *p<0.05 vs ovalbumin-DC treat- ment; **p<0.05 vs CY-treatment only

Journal: Diabetologia

Article Title: Autoantigen-specific protection of non-obese diabetic mice from cyclophosphamide-accelerated diabetes by vaccination with dendritic cells.

doi: 10.1007/s00125-003-1199-0

Figure Lengend Snippet: Fig. 8A–C. Vaccination with insulin-pulsed NOD-DC1 cells induced a change in Th1/Th2 balance. At day 10 after CY- treatment IL-10 and IFN-γ expression was measured. (A) Cy- tokine expression in the pancreas detected by Light Cycler RT- PCR. The number of mRNA copies are expressed per 1×106 β- actin molecules. (B) Cytokine secretion in culture supernatants after stimulation of 3×106 splenocytes with 50 µg/ml insulin for 3 days. (C) ELISPOT analysis of insulin-specific T-cell re- activity in NOD mice following vaccination with NOD-DC-1 cells. The frequency of IFN-γ spot-forming colonies (SFC) per well in response to 50 µg/ml insulin were calculated from trip- licates of individual mice. *p<0.05 vs ovalbumin-DC treat- ment; **p<0.05 vs CY-treatment only

Article Snippet: T-cell response to insulin was also analysed by ELISPOT using IFN-γ ELISPOT development modules (R&D Systems).

Techniques: Expressing, Reverse Transcription Polymerase Chain Reaction, Enzyme-linked Immunospot, Activity Assay

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canine ifnγ elispot development module (R&D Systems)

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1) Product Images from "Rabies vaccination induces a CD4 + T EM and CD4 + CD8 + T EMRA T H 1 phenotype in dogs"

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